I guess it depends on the cards that you run. Remember that the middle slot is PCI-E 1.1. If you run a PCI-E 2.0 card in the middle slot, there
MIGHT be a difference. I could check it out for you with heavily OCed GTX 260s in SLI with vantage.
Edit: It looks like it does affect the performance. I lost 2k in vantage.
This is with my GTX 260s in slots 1 and 3.
and this is with them on 1 and 2
Also, when I put the cars on 1 and 2, the responsiveness of the system was pretty bad too. There was a lot of stuttering in vista. I have 4 raptors in raid 0+1 seagate+1 DVD drive all on the MCP with 1.55V. Considering that the middle slot is connected to the MCP, the MCP might require more volts if you have something like me(with all 6 sata ports occupied).
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