Just to clarify, I've used WinSetupFromUSB dozens of times, and it have never failed for me. I've never tried making a W7 AIO ISO, will try it after I get SP1.
That's strange, this is the only SW that actually works for me with anything XP, 2003, Vista, W7..
For single Windows ISO, quick format in Windows first, NTFS.
Then start WinSetupFromUSB and add the ISO you want.
No need to touch the MBR controls etc. for me anyway.
Make sure you pick the right choice during boot countdown.
I can also recommend
Unetbootin for any Windows 6.x, but with 5.x it's more of a hit and miss. Maybe it got better, dunno.
Are you sure your USB memory works well with booting? Also, try format to FAT32 if NTFS fails, sometimes it makes a difference in Unetbootin + Windows 5.x, maybe in WinSetupFromUSB too.
Unlike MS official USB installer, both WinSetupFromUSB and Unetbootin works with modified ISO's (nlite, vlite...), and also works with Linux and other bootable ISO's.
I dunno if adding two ISO's with WinSetupFromUSB is a good idea, after reading the guide...
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